【作者】 王正春; 蔣海艷; 楊笑笑; 李月文; 李繼暉; 鄭凌凌;
【Author】 WANG Zheng-chun1,2,JIANG Hai-yan1,YANG Xiao-xiao2,LI Yue-wen1,LI Ji-hui3,ZHENG Ling-ling3(1.Forestry Research Institute of Chongqing,Chongqin 400036;2.Southwest University,Chongqin 400715; 3.Pengshui Autonomous County Forestry Science and Technology Promotion Station,Pengshui Sichuan 409600)
【機構(gòu)】 重慶市林業(yè)科學(xué)研究院; 西南大學(xué)資源環(huán)境學(xué)院; 彭水自治縣林業(yè)科技推廣站;
【摘要】 通過2種不同的培養(yǎng)基對彭水2種野生牛肝菌子實體進行分離、培養(yǎng),同時,進行液體培養(yǎng),培養(yǎng)的菌絲經(jīng)過ITS鑒定,放入-80℃超低溫冰箱中保藏。結(jié)果表明,2種牛肝菌菌絲均從組織塊表面邊緣生長,菌絲潔白、小,不向周圍培養(yǎng)基生長,老化速度較快,老化過程中菌絲減少,最后變?yōu)楣腆w狀物,并且分泌褐色水珠;液體培養(yǎng)菌絲體生長緩慢,培養(yǎng)時間較長,菌絲球小、少;菌絲球經(jīng)過鑒定后,確定2種牛肝菌均為美味牛肝菌。
【Abstract】 Through two different culture medium,two wild Boletus edulis were seperated and cultured,and training hyphae were identificated by ITS,which were stord in the refrigerator of-80℃.The results showed that the two Boletus edulis hyphe liver tissue were growth from mass surface edge,and the hyphe were white and small.The hyphe didn’t grow around the culture medium,and aging faster.The mycelia by liquid culture grew slowly,and the ball was small.Hyphae ball by determining were Boletus edulis.
【關(guān)鍵詞】 重慶; 彭水; 美味牛肝菌; 分離培養(yǎng); 液體培養(yǎng); ITS鑒定; 保藏;
【Key words】 Chongqing; Pengshui; Boletus edulis; Separation training; Liquid culture; ITS identification; Preservation;
【基金】 重慶市科技攻關(guān)項目“彭水縣野生牛肝菌人工馴化關(guān)鍵技術(shù)研究”(CSTC,2010AC1111);“武陵山區(qū)食用菌良種高效栽培試驗示范”(CSTC,2010AB1047);重慶市林科院科技計劃項目“萬州區(qū)老林村林下食用菌栽培示范”、“重慶桉樹、竹林林下種植模式技術(shù)研究”
【分類號】S646.3