【作者】 李冠喜； 華國(guó)棟； 王多明； 魏良志；

【Author】 LI Guanxi1,2,HUA Guodong2,WANG Duoming2,WEI Liangzhi3 1Lianyungang City Academy of Agricultural Sciences,Lianyungang,Jiangsu 222006,China; 2College of Forest Resources and Environment,Nanjing Forestry University,Nanjing,Jiangsu 210037,China; 3Xinba Town Agricultural Technology Generalization Center of Haizhou District,Lianyungang,Jiangsu 222001,China

【機(jī)構(gòu)】 連云港市農(nóng)業(yè)科學(xué)院； 南京林業(yè)大學(xué)森林資源與環(huán)境學(xué)院； 連云港市海州區(qū)新壩鎮(zhèn)農(nóng)業(yè)技術(shù)推廣中心；

【摘要】 采用同核體單孢雜交育種技術(shù),以低溫高產(chǎn)的雙1菌株和高溫低產(chǎn)的雙5菌株為親本,選育雙孢蘑菇優(yōu)良新品種。通過(guò)擔(dān)孢子分離和同核體的鑒別,獲得同核體菌株共20個(gè),同核體間配對(duì)雜交組合99個(gè),獲得10個(gè)雜交異核體菌株;經(jīng)過(guò)菇房、出菇地棚栽培和出菇大棚生產(chǎn)性試驗(yàn),篩選出高產(chǎn)優(yōu)質(zhì)雜交菌株Z1。

【Abstract】 Monokaryotic single-spore isolates obtained from the high yield Shuang 1 strain and the low yield,high temperature Shuang 5 strain of Agaricus bisporus were used for breeding new high yielding,elevated temperature tolerant hybrids.Ninety-nine pairings were obtained from 20 monokaryotic spore isolates(11 from Shuang 1 and nine from Shuang 5,identified on the basis of isozyme profiles),and ten dikaryotic hybrids were obtained.After three cultivation cycles(carried out on a laboratory-scale,in 3 m2...

【關(guān)鍵詞】 雙孢蘑菇； 單孢雜交育種； 雜交；

【Key words】 Agaricus bisporus； cross-breeding using single spore isolates； hybridization；

【基金】 江蘇省農(nóng)業(yè)三項(xiàng)工程項(xiàng)目[編號(hào):sx(2009)133];連云港市農(nóng)業(yè)科技成果示范計(jì)劃項(xiàng)目(編號(hào):SF0902)的部分研究?jī)?nèi)容

【分類號(hào)】S646.11