【作者】 章慧; 張勁松; 賈薇; 馮娜; 唐傳紅; 楊焱; 唐慶九; 劉艷芳; 潘迎捷;
【Author】 ZHANG Hui1,2,ZHANG Jingsong1,JIA Wei,FENG Na,TANG Chuanhong,YANG Yan TANG Qingjiu,LIU Yanfang,PAN Yingjie2 1National Engineering Research Center of Edible Fungi;Shanghai Key Laboratory of Agricultural Genetics and Breeding;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China; 2Shanghai Ocean University,Shanghai 201306,China
【機(jī)構(gòu)】 農(nóng)業(yè)部南方食用菌資源利用重點(diǎn)實(shí)驗(yàn)室國家食用菌工程技術(shù)研究中心上海市農(nóng)業(yè)遺傳育種重點(diǎn)開放實(shí)驗(yàn)室上海市農(nóng)業(yè)科學(xué)院食用菌研究所; 上海海洋大學(xué);
【摘要】 采用超臨界CO2(SFE-CO2)技術(shù)萃取靈芝(Ganoderma lucidum)子實(shí)體中的三萜成分,通過單因素試驗(yàn)和正交試驗(yàn)確定最佳條件為:子實(shí)體粒度10目、夾帶劑95%乙醇、壓力35Mpa、溫度40℃、時間2.5h、CO2流量35g/min、夾帶劑體積:子實(shí)體重6∶1(mL/g),萃取物的得率為2.42%,總?cè)频牡寐蕿?.98%,萃取物中三萜的含量為40.5%。與傳統(tǒng)的醇提法相比,SFE-CO2法中粗提物和總?cè)频妮腿÷事缘?但粗提物中三萜的含量較高,且HPLC研究表明,SFE-CO2的粗提物中三萜的種類較多。
【Abstract】 Single parameter and orthogonal tests were used to determine the optimal conditions for extracting triterpenes from Ganoderma lucidum fruit bodies using supercritical CO2 extraction(SFE-CO2).Highest extraction rates were achieved when fruit bodies were pulverized to 10 mesh particle size,an entrainer(ethanol) concentration of 95%,35 Mpa extraction pressure,40 ℃,2.5 h extraction time,a carbon dioxide flow rate of 35 g/min),and an entrainer:G.lucidum fruit body weight ratio of 6∶1(mL∶g).Under thes...
【關(guān)鍵詞】 靈芝三萜; 超臨界CO2萃??; 醇提法;
【Key words】 Ganoderma lucidum; triterpene; supercritical CO2 extraction;
【基金】 上海市農(nóng)委食用菌深加工關(guān)鍵技術(shù)研究與示范[編號:農(nóng)科攻字2008(8-2)]的部分研究內(nèi)容
【分類號】R284.1