鵝膏菌屬真菌RAPD分析及親緣關(guān)系的研究

陳作紅　張志光　張?zhí)鞎?p>

摘要：本文對采自湖南莽山的26種鵝膏菌屬（Amanita）真菌進(jìn)行了隨機(jī)擴(kuò)增多態(tài)性DNA （RAPD）分析，40個隨機(jī)引物中篩選出擴(kuò)增效果較好的6個引物，每個引物能產(chǎn)生1～10條DNA條帶，獲得的RAPD譜帶清晰并呈現(xiàn)多態(tài)性O(shè)PG15、OPH04兩個引物擴(kuò)增的RAPD譜帶能將26種鵝膏菌完全區(qū)分開來，通過6個引物的RAPD分析獲得的平均相似性系數(shù)表明種與種之間的相關(guān)系數(shù)在20D60%之間，平均鏈鎖聚類分析可將26種鵝膏菌分為二大類，且一些具菌環(huán)和苞狀菌托的種類聚在一起，與形態(tài)分類基本相吻合。

關(guān)鍵詞：鵝膏菌屬， RAPD， 親緣關(guān)系

中圖分類號：Q939.5　文獻(xiàn)標(biāo)識碼：A

文章編號：1007-3515（2000）01-0051-0055

STUDY ON RELATIONSHIP AMONG SPECIES OF

AMANITA USING RAPD

CHEN Zuo-Hong ZHANG Zhi-Guang ZHANG Tian-Xiao

(College of Life Science, Hunan Normal University, Changsha, 410081)

ABSTRACT: Random amplified polymorphic DNA (RAPD) was used in analysing the relationship among twenty-six speices of Amanita from Mangshan, Hunan. Of 40 primers, 6 ones generated reproducible RAPD profiles, and each one produced 1-10 bands. Two primers (OPG15 and OPH04) could differentiate 26 species of Amanita by its RAPD profiles. The similarity coefficients obtained from profiles generated by six primers among Amanita were about 20-60%. A dendrogram was constructed using average linkage clustering for clustering analysis. It indicated that there were two groups in 26 species and some species with ring and membranous volva clustered a group which coincided with morphological classification.

KEY WORDS: Amanita, RAPD, Relationship

國家科委重點(diǎn)課題資助項(xiàng)目93（7）-03

陳作紅（湖南師范大學(xué)生命科學(xué)學(xué)院　長沙　410081）

張志光（湖南師范大學(xué)生命科學(xué)學(xué)院　長沙　410081）

張?zhí)鞎裕ê蠋煼洞髮W(xué)生命科學(xué)學(xué)院　長沙　410081）

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