食用菌遺傳轉(zhuǎn)化研究進(jìn)展

Research Advances in Genetic Transformation of Edible Fungi

郭麗瓊　陳守才　林俊芳　

摘　要：食用菌遺傳轉(zhuǎn)化的方法主要有PEG法、電激法、基因槍法、限制酶介導(dǎo)法、農(nóng)桿菌介導(dǎo)法;采用的啟動(dòng)子有fas啟動(dòng)子和gpd啟動(dòng)子;采用的篩選標(biāo)記有營養(yǎng)缺陷型標(biāo)記、抗生素抗性篩選標(biāo)記、除草劑和殺菌劑抗性篩選標(biāo)記、代謝產(chǎn)物抗性篩選標(biāo)記.本文綜述了這些遺傳轉(zhuǎn)化方法以及啟動(dòng)子、篩選標(biāo)記的最新研究進(jìn)展.

關(guān)鍵詞：食用菌;遺傳轉(zhuǎn)化;限制酶介導(dǎo)法;農(nóng)桿菌介導(dǎo)法;啟動(dòng)子;篩選標(biāo)記

分類號：S646.032　文獻(xiàn)標(biāo)識碼：A

文章編號：1005-9873(2001)04-47-53

基金項(xiàng)目：國家自然科學(xué)基金資助項(xiàng)目(39960050)的一部分

作者簡介：林俊芳責(zé)任作者

作者單位：郭麗瓊（中國熱帶農(nóng)業(yè)科學(xué)院熱帶作物生物技術(shù)國家重點(diǎn)實(shí)驗(yàn)室,海南海口571101）　

　　　　　陳守才（中國熱帶農(nóng)業(yè)科學(xué)院熱帶作物生物技術(shù)國家重點(diǎn)實(shí)驗(yàn)室,海南?？?71101）　

　　　　　林俊芳（中國熱帶農(nóng)業(yè)科學(xué)院熱帶作物生物技術(shù)國家重點(diǎn)實(shí)驗(yàn)室,海南?？?71101）　

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