地高辛標(biāo)記核酸探針的標(biāo)記方法及其在食用菌研究領(lǐng)域的應(yīng)用
A Review on the Labeling Method of DIG-Labeled Nucleic-Acid-Probe and Its Application in the Field of Edible Fungi
葛丹 陳明杰 曹文偉
摘 要：綜述了非放射性地高辛(DIG)標(biāo)記系統(tǒng)的原理和主要特點,介紹了地高辛標(biāo)記核酸探針的主要標(biāo)記方法,影響探針標(biāo)記方法選擇的因素,標(biāo)記探針的顯色檢測方法及其在食用菌研究領(lǐng)域的應(yīng)用.
關(guān)鍵詞：核酸探針;地高辛標(biāo)記;分子雜交
分類號：S646.01;S124.2 文獻(xiàn)標(biāo)識碼：A
文章編號：1005-9873(2002)02-50-06
作者簡介：陳明杰,本文通訊作者.
作者單位：葛丹（遼寧師范大學(xué)生命科學(xué)學(xué)院,大連,116029）
陳明杰（農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室,上海市農(nóng)業(yè)遺傳育種重點實驗室,上海市農(nóng)業(yè)科學(xué)院食用菌研究所,上海,201106）
曹文偉（遼寧師范大學(xué)生命科學(xué)學(xué)院,大連,116029）
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