針層孔菌屬(Phellinus)中藥用真菌的研究概述 陶美華 章衛(wèi)民 鐘韓 李浩華 摘 要:敘述了國內(nèi)外針層孔菌屬(Phellinus)中主要的藥用真菌在人工栽培、發(fā)酵培養(yǎng)、化學(xué)成分分析和藥理作用方面的研究現(xiàn)狀,并簡要分析了當(dāng)前針層孔菌研究中存在的一些問題. 關(guān)鍵詞:針層孔菌屬;人工栽培;發(fā)酵培養(yǎng);化學(xué)成分;多糖;藥理作用 分類號:S576.301;R151.3 文獻(xiàn)標(biāo)識(shí)碼:A 文章編號:1005-9873(2005)04-0065-08 Review on the Research of Medicinal Fungi in the Genus Phellinus TAO Mei-hua ZHANG Wei-min ZHONG Han LI Hao-hua 基金項(xiàng)目:廣東省科技攻關(guān)項(xiàng)目"抗腫瘤活性物質(zhì)PLP的提取、分離純化及應(yīng)用研究"(編號:2003C104044)的部分內(nèi)容 作者簡介:陶美華(1978-),女,2004年畢業(yè)于湖南農(nóng)業(yè)大學(xué),碩士,主要從事藥用真菌研究與開發(fā),發(fā)表論文6篇. 作者單位:陶美華(廣東省微生物研究所,廣州,510070) 章衛(wèi)民(廣東省微生物研究所,廣州,510070) 鐘韓(廣東省微生物研究所,廣州,510070) 李浩華(廣東省微生物研究所,廣州,510070) 參考文獻(xiàn): [1]Dai YC.Phellinus sensu lato (Aphyllophorales,Hymenochaetaceae) in East Asia[J].Acta Bot Fennici,1999,166:111~ 115. [2]Hata T.Recent advances in medical and veterinary mycology[M].Baltimore:University Park Press,1997.299. [3]Song CH,Moon HH,Ryu CH.Artificial cultivation of Phellinus linteus [J] Korean J Mycol,1997,25:130~ 132. [4]Baea JS,Jang KH,Yim H,et al.Polysaccharides isolated from Phellinus gilvus inhibit melanoma growth in mice[J].Cancer Letters,2005,218:43~52. [5]李國俊,吳國用,崔基成,等.裂蹄木層孔菌菌絲培養(yǎng)及其應(yīng)用研究[J].中國食用菌,1998,17(5):27~28. [6]楊全,李艷輝,嚴(yán)寒靜,等.藥用真菌桑黃液體發(fā)酵工藝的研究[J].廣東藥學(xué)院學(xué)報(bào),2004,20(3):212~215. [7]樊錦艷,王秋穎,薛梅,等.桑黃胞外多糖生產(chǎn)培養(yǎng)基的初步研究[J].食品科技,2004,2:93~95. [8]Kim DH,Yang BK,Jeong SC,et al.Production of a hypoglycemic extracellular polysaccharide from the submerged culture of the mushroom,Phellinus linteus [J].Biotechnology Letters,2001,23:513~517. [9]Kim SW,Hwang HJ,Park JP,et al.Mycelial growth and exo-biopolymer production by submerged culture of various edible mushrooms under different media[J ].Letters in Applied Microbiology,2002,34:56~61. [10]Hwang HJ,Kim SW,Choi JW,et al.Production and characterization of exopolysaccharides from submerged culture of Phellinus linteus KCTC 6190[J].Enzyme and Microbial Technology,2003,33:309~319. [11]Hwang HJ,Kim SW,Xu CP,et al.Morphological and rheological properties of the three different species of basidiomycetes Phellinus in submerged cultures[J ].Journal of Applied Microbiology,2004,96:1296~ 1305. [12]Hwang HJ,Kim SW,Xu CP,et al.Production and molecular characteristics of four groups of exopolysaccharides from submerged culture of Phellinus gilvus [J ].Journal of Applied Microbiology,2003,94:708~719. [13]白日霞,陳華君.堿提水溶針裂蹄多糖R1的研究[J].天然產(chǎn)物研究與開發(fā),1995,7(3):41~45. [14]Kim GY,Park HS,Nam BH,et al.Purification and characterization of acidic proteo-heteroglycan from the fruiting body of Phellinus linteus (Berk.&M.A.Curtis)Teng[J].Bioresource Technology,2003,89:81 ~87. [15]Song KS,Cho SM,Lee JH,et al.B-lymphocyte stimulating polysaccharide from mushroom Phellinus linteus[J].Chemical and Pharmaceutical Bulletin,1995,43:2105~2108. [16]Nakamura T,Matsugo S,Uzuka Y,et al.Fractionation and anti-rumor activity of the mycelia of liquid cultured Phellinus linteus [J].Biosci Biotechnol Biochem,2004,68(4) :868~872. [17]劉金榮,江發(fā)濤,李艷,等.藥用真菌桑黃甾類成分的提取和鑒定[J].農(nóng)墾醫(yī)學(xué),1998,20(3):141. [18]莫順燕,楊永春,石建功.桑黃化學(xué)成分研究[J].中國中藥雜志,2003,28(4):339 341. [19]莫順燕,楊永春,石建功.桑黃黃酮A和B的分離與合成[J].化學(xué)學(xué)報(bào),2003,61(7):1161~1163. [20]Moon DC,Hwang KH,Choi KR,et al.Constituents of ceramide of a native mushroom,Phellinus ribis in Korea[J].Analytical Science and Technology,1994,7:547~ 554. [21]Ayer WA,Muir DJ,Chakravarty P.Phenolic and other metabolites of Phellinus pini,a fungus pathogenic to pine[J].Phytochemistry,1996,42:1321~1324. [22]Ikekawa T,Nakanishi M,Uehara N,et al.Antitumor action of some basidiomycetes,especially Phellinus linteus [J].Gann,1968,59:155~157. [23]溫克,陳勁,李紅,等.桑黃等四種抗癌藥物抗癌活性比較[J].吉林大學(xué)學(xué)報(bào)(醫(yī)學(xué)版),2002,28(3):247~249. [24]Ajith TA,Janardhanan KK.Cytotoxic and antitumor activities of a polypore macrofungus,Phellinus rimosus(Berk) Pilat[J].Journal of Ethnopharmacology,2003,84:157~ 162. [25]Chung KS,Kim HS,Kim KY,et al.Effect of Kp,an antitumor protein polysaccharide from mycdial culture of Phellinus linteus on the humoral immune response of rumor bearing ICR mice to sheep red blood cells[J].Archives of Pharmaceutical Research,1993,16:336~338. [26]Sasaki T,Arai Y,Ikekawa T,et al.Antitumor polysaccharides from some polyporaceae,Ganoderma applanatum (Pers.) Pat and Phe1llinus linteus (Berk.et Curt)Aoshima[J].Chem Pharm Bull(Tokyo),1971,19(4) :821~826. [27]Han SB,Lee CW,Jeon YJ,et al.The inhibitory effect of polysaccharides from Phellinus linteus on tumor growth and metastasis[J].Immunopharmacology,1999,41:157~ 164. [28]Shon YH,Nam KS.Antimutagenicity and induction of anticarcinogenic phase Ⅱ enzymes by basidiomycetes[J ].Journal of Ethnopharmacology,2001,77:103~ 109. [29]Cho JH,Cho SD,Hu H,et al.The roles of ERK 1/2 and p38 MAP kinases in the prevention mechanism of mushroom Phellinus linteus against the inhibition of gap junctional intercellular communication by hydrogen peroxide[J ].Carcinogenesis,2002,23:1164~1169. [30]Song YS,Kim SH,Sa JH,et al.Anti-angiogenic,antioxidant and xanthine oxidase inhibition activities of the mushroom Phellinus linteus [ J].Journal of Ethnopharmacology,2003,88:113~ 116. [31]Kim HM,Han SB,Oh GT,et al.Stimulation of humoral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus [J].International Journal of I mmunopharmacology,1996,18(5):295~303. [32]Kim GY,Park SK,Lee MK,et al.Proteoglycan isolated from Phellinus linteus activates murine B lymphocytes via protein kinase C and protein tyrosine kinase[J].International Immunopharmacology,2003,3:1281~ 1292. [33]Park SK,Kim GY,Lim JY,et al.Acidic polysaccharides isolated from Phellinus linteus induce phenotypic and functional maturation of murine dendritic cells [J ].Biochemical and Biophysical Research Communications,2003,312:449~458. [34]Jeon TI,Hwang SG,Lim BO,et al.Extracts ofPhellinus linteus grown on germinated brown rice suppress liver damage induced by carbon tetrachloride in rats[J].Biotechnol Lett,2003,25(24) :2093~2096. [35]Kim SH,Lee HS,Lee S,et al.Mycelial culture of Phellinus linteus protects primary cultured rat hepatocytes against hepatotoxins[J ].Journal of Ethnopharmacology,2004 ,95(2~ 3) :367 ~ 372. [36]張萬國,胡晉紅,蔡溱,等.桑黃對四氯化碳致大鼠肝損傷的保護(hù)作用[J].中國藥房,2003,14(5):267~269. [37]Ajith TA,Janardhanan KK.Antioxidant and antihepatotoxic activities of Phellinus rimosus (Berk) Pilat[J].Journal of Ethnopharmacology,2002,81:387~ 391. [38]Kim SH,Song YS,Kim SK,et al.Anti-inflammatory and related pharmacological activities of the n-BuOH subfraction of mushroom Phellinus linteus [J ].Journal of Ethnopharmacology,2004,93:141~ 146. [39]Ajith TA,Jose N,Janardhanan KK.Amelioration of cisplatin induced nephrotoxicity in mice by ethyl acetate extract of a polypore fungus,Phellinus rimosus [ J ].J Exp Clin Cancer Res,2002,21 (2):213 ~ 217. [40]Kim GY,Kim SH,Hwang SY,et al.Oral administration of proteoglycan isolated from Phellinus linteus in the prevention and treatment of collagen-induced arthritis in mice[J].Biological and Pharmaceutical Bulletin,2003,26:823~ 831. [41]Hwang HJ,Kim SW,Lim JM,et al.Hypoglycemic effect of crude exopolysaccharides produced by a medicinal mushroom Phellinus baumii in streptozotocin-induced diabetic rats [J ].Life Sciences,2005,76 (26):3069~3080. [42]Liu F,Ooi VE,Chang ST.Free radical scavenging activities of mushroom polysaccharide extracts [J].Life Sci,1997,60:763~771. 食用菌學(xué)報(bào) 2005年第4期-HTM文件 No.14