Glucose (葡萄糖) 10g Yeast extract (酵母膏) 10g
Starch (淀粉) 10g NaCl 5g
CaCO3 3g Agar (瓊脂) 20g
Distilled water (蒸餾水) 1L pH 7.0
30. Wheat Bran Medium (麩皮培養(yǎng)基)
Wheat bran (麩皮) 36g (NH4)2HPO4 10g
K2HPO4 0.2g MgSO4。7H2O 0.1g
Agar (瓊脂) 20g Distilled water (蒸餾水) 1L
pH 7.0
31. Yeast Sporulation Medium (酵母菌產(chǎn)生子囊孢子培養(yǎng)基)
(1) Kleyn Medium
KH2PO4 0.012% K2HPO4 0.02%
Sodium acetate (醋酸鈉) 0.5% Glucose (葡萄糖) 0.062%
NaCl 0.062% Peptone (蛋白胨) 0.25%
Biotin (生物素) 2μg% Agar (瓊脂) 2%
Trace element solution (混合鹽類) 1ml/100ml
[Note]: Trace element solution (混合鹽類溶液): MgSO4.7H2O 0.4%, NaCl 0.4%, CuSO4.5H2O 0.002%, MnSO4.4H2O 0.2%, FeSO4.4H2O 0.2%
(2) Gorodkowa Medium
Glucose (葡萄糖) 0.1% Peptone (蛋白胨) 1%
NaCl 0.5% Agar (瓊脂) 2%
(3) McClary Medium
Glucose (葡萄糖) 0.1% KCl 0.18%
Yeast juice (酵母汁) 0.25% Sodium actate (醋酸鈉) 0.82%
Agar (瓊脂) 1.5%
32. BPY Medium (BPY 培養(yǎng)基)
Beef extract (牛肉膏) 5g Prptone (蛋白胨) 10g
NaCl 5g Yeast extract (酵母膏) 5g
Glucose (葡萄糖) 5g Agar (瓊脂) 1-2%
Distilled (蒸餾水) 1000ml pH 7.0
適用范圍:大腸埃希氏菌(大腸桿菌)、惡臭假單胞菌
33. LB Medium (LB 培養(yǎng)基)
Yeast extact (酵母膏) 5g Peptone (蛋白胨) 10g
NaCl 10g Agar (瓊脂) 1-2%
Distilled water (蒸餾水) 1000ml pH 7.0
適用范圍:大腸埃希氏菌(大腸桿菌)
34. Penssay Broth (Penssay 肉湯)
Beef extract (牛肉膏) 1.5g Yeast extract (酵母膏) 1.5g
Tryptone (胰蛋白胨) 5g NaCl 3.5g
Glucose (葡萄糖) 1g K2HPO4.3H2O 4.8g
Distilled water (蒸餾水) 1000ml pH 7.2
35. Blond Agar (血瓊脂培養(yǎng)基)
Peptone (蛋白胨) 10g Glucose (葡萄糖) 2g
NaCl 5g NaH2PO4 2.5g
Sodium citrate (檸檬酸鈉) 5g Brain infusion (腦浸汁) 800ml
Pig heart infusion (豬心浸) 200ml Sleep Blood (羊血) 10%
pH 7.2
[Note]:Preparation of pig brain and pig heart infusion:
(1) Smash up 100g fresh pig heart (without fat and blood vessel).Add distilled water up to 1000ml;
(2) Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml;
(3) Keep the two infusions at 50℃ for 1 hr , then boil for 15 minutes;
(4) Cool and filtrate through gauze . [注]豬心和豬腦浸汁法:(1)新鮮豬心除去心頭和脂肪,攪碎100克加蒸餾水至1000毫升;(2)新鮮豬腦除去血管,取200克加蒸餾水至1000毫升;(3)50℃保溫1小時(shí),然后煮開15分鐘;(4)冷卻后用紗布過(guò)濾備用。
36. Cellulosedecomposing Bacteria Synthetic Medium (纖維細(xì)菌合成培養(yǎng)基)
NaCl 6g MgSO4.7H2O 0.1g
KH2PO4 0.5g CaCl2 0.1g
K2HPO4 2.0g Yeast extract (酵母膏) 1.0g
(NH4)2SO4 2.0g Distilled water (蒸餾水) 1000ml
Filter paper strip: one strip (濾紙條:一片) pH 7.0-7.4
[Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl Cellulose 2% Agar 2%. (斜面培養(yǎng)基去濾紙條用2%瓊脂及2%羧甲基纖維素鈉 CMCNa )
37. Clostridium Pasreurianum Synthetic Medium (巴氏梭狀芽孢桿菌合成培養(yǎng)基)
Glucose (葡萄糖) 1% MnSO4 0.001%
KH2PO4 0.05% FeSO4 0.001%
K2HPO4 0.05% Yeast extract(酵母膏) 0.1%
MgSO4.7H2O 0.02% Peptone (蛋白胨) 0.01%
NaCl 0.001% CaCO3 0.5%
Distille water (蒸餾水) 100% pH 7.0
38. Yeast Extract-Medium Extract Agar Ⅱ (酵母膏、麥芽膏瓊脂 Ⅱ)
Yeast extract (酵母膏) 4g Mall extract (麥芽膏) 10g
Glucose (葡萄糖) 4g Agar (瓊脂) 17g
Distilled water (蒸餾) 1000ml pH 7.3
39. Oatemeal Agar (燕麥粉瓊脂)
Oatemeal(infusion)[燕麥粉浸液] 20g Agar (瓊脂) 18%
Trace element solution (跡量鹽溶液)1ml
Distilled water up to (用蒸餾水補(bǔ)充至1000毫升) 1000ml pH 7.2
[Note]:Trace element solution (跡量鹽溶液):
FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g
ZnSO4.7H2O 0.1g Distilled water (蒸餾水) 100ml
40. Calcium Malate Agar (蘋果酸鈣瓊脂)
Calium malate (蘋果酸鈣) 10g K2HPO4 0.5g
NH4Cl 0.5g Glycerol (甘油) 10g
Agar (瓊脂) 15g Distilled water (蒸餾水) 1000ml
41. Thiobacillus Ferrooxidans Medium (氧化亞鐵硫桿菌培養(yǎng)基)
(NH4)2SO4 0.15g KH2PO4 0.05g
KCl 0.05g MgSO4.7H2O 0.5g
Ca(NO3)2.4H2O 0.01g FeSO4.7H2O 50g
Distilled water (蒸餾水) 1000ml pH 2.0
42. Thiobacillus Thiooxidans Medium (氧化硫硫桿菌培養(yǎng)基)
(NH4)2SO4 0.3g KH2PO4 3-4g
CaCl2 (anhydrous) [無(wú)水] 0.25g MgSO4.7H2O 0.5g
FeSO4.7H2O 0.001g Distilled water (蒸餾水) 1000ml
pH 3.5-4
43. Plasma Substitute Medium (代血漿培養(yǎng)基)
Sucrose (蔗糖) 12.5-15% Peptone (蛋白胨) 0.5%
KH2PO4 0.03% Na2HPO4.12H2O 0.14%
pH 7.0-7.2
44. Skim Milk Medium (脫脂牛奶培養(yǎng)基) ATCC 183
脫脂牛奶 100g 去離子水 1000ml
適用范圍:保加利亞乳桿菌、嗜熱乳酸鏈球菌
45. Pea Agar (豌豆瓊脂)
Glucose (葡萄糖) 1% Peptone (蛋白胨) 0.5%
NaCl 0.5% Agar (瓊脂) 2.0%
Pea infusion (counted by NH2) [豌豆浸汁(以NH2計(jì))] 12-14 mgM100
pH (before sterilization) [消前] 7.2-7.5
[Note]: Preparetion of pea infusion (豌豆浸汁的制法) :
Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust pH to 4.3-4.5 by using of sulphuric acid. Add 30ml of chloroform for antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge in order to avoid that the stopper fall off due to gas pressure. Place the bottle at 37℃ for 7-10 days. Filter the infusion, then boil the filtrate to escape the chloroform. Filter again Distribution and autoclave at 120℃ for 30 minutes. Store at cool contition . (稱取白豌豆900克,置于5公升廣口瓶中,加自來(lái)水3公升,用硫酸調(diào)pH至4.3-4.5,再加氯仿30毫升(防腐),加膠塞并用紗布扎口(防止沖脫),放37℃恒溫室7-10天。取出用濾紙過(guò)濾,濾液煮沸20分鐘,以除氯仿,并再次以濾紙過(guò)濾,除沉淀。濾液分裝,于120℃30分鐘滅菌。后冷藏備用。)
The standard quality of pea infusion as following (豌豆浸汁質(zhì)量標(biāo)準(zhǔn)):
Aminonitrogen(氨基酸): 160-200 mgM100ml
Soluble phosphoruse(溶磷): 400-600 μgMml
Color(色澤): auratus,transparent (金黃、透明)
46. Wheat Bran Medium (麥麩瓊脂)
Wheat bran (麥麩) 3.6% (NH4)2HPO4 0.3%
K2HPO4 0.02% MgSO4.7H2O 0.01%
Agar (瓊脂) 2% pH 7.0
適用范圍:金霉素鏈霉菌、紅霉素鏈霉菌
47. Glucose Yeast Extract Agar (葡萄糖、酵母膏瓊脂)
Glucose (葡萄糖) 10g Yeast extract (酵母膏) 10g
Water (水) 1000ml Agar (瓊脂) 15g
pH 7.2
48. MMN Medium (MMN培養(yǎng)基)
CaCl2 0.05g MgSO4 0.15g
NaCl 0.025g FeCl3 (1%) 1.2ml
KH2PO4 0.5g Vitamib B1 (硫胺素) 100μg
(NH4)2HPO4 0.25g Wort (12 Be′) 100ml
Glucose (葡萄糖) 10-15g Citric acid (檸檬酸) 0.2g
Distilled water (蒸餾水) 900ml pH 5.5 (左右)
適用范圍:蜜環(huán)菌
49. Starch Agar (淀粉瓊脂)
Soluble starch (可溶性淀粉) 10g NaNO3 1g
MgCO3 1g K2HPO4 0.3g
NaCl 0.5g Agar (瓊脂) 20g
Distilled water (蒸餾水) 1000ml
50. 5 Be′Wort Agar (5 Be′麥芽汁瓊脂)
適用范圍:園弧青霉、少根根霉、日本根霉、爪哇根霉
51. 5 Be′Koji Extract Agar (5 Be′米曲汁瓊脂)
適用范圍:華根霉、三寶壟根霉
52. 發(fā)根農(nóng)桿菌培養(yǎng)基(YEB)
Beef extract (牛肉浸膏) 5g/L Yeast extract (酵母膏) 1g/L
Peptone (蛋白胨) 5g/L Sucrose (蔗糖) 5g/L
MgSO4.7H2O 0.4g/100ml Agar (瓊脂) 1.5g/100ml
pH 7.4
適用范圍:青枯假單胞菌
53. 青枯菌培養(yǎng)基 (Tm)
Peptone (蛋白胨) 10g Casein (干酪素水解物產(chǎn)) 1g
Glucose (葡萄糖) 5g Ddhro (純水) 1000ml
Agar(瓊脂) 15g pH 7.0
54. YM (Yeast Malt Agar)
酵母膏 3.0g 麥芽汁 3.0g
蛋白胨 5.0g 瓊脂 20.0g
蒸餾水 1000ml
55. ISP 4
可溶性淀粉 10.0g K2HPO4 1.0g
MgSO4.7H2O 1.0g NaCl 1.0g
(NH4)2SO4 2.0g CaCO3 2.0g
FeSO4.7H2O 0.001g MnCl2.7H2O 0.001g
瓊脂 20.0g 蒸餾水 1000ml
56. Trypton Soy Agar
胰蛋白胨 17.0g 大豆胨 3.0g
葡萄糖 2.5g 瓊脂 20.0g
NaCl 5.0g K2HPO4 2.5g
蒸餾水 1000ml
57. NA (Nutrient Agar)
牛肉膏 3.0g 蛋白胨 5.0g
瓊脂 20.0g 蒸餾水 1000ml
pH 6.6-7.0
58. 甘露(糖)醇瓊脂 (ATCC 1)
酵母膏 5.0g 蛋白胨 3.0g
甘露(糖)醇 25.0g 瓊脂 15.0g
蒸餾水 1000ml
59. Marine瓊脂 2216 (ATCC 2)
蛋白胨 5.0g 酵母膏 1.0g
檸檬酸鐵 0.1g 溴化鉀 0.08g
NaCl 19.45g MgCl2 8.8g
Na2SO4 3.24g CaCl2 1.8g
KCl 0.55g NaHCO3 0.16g
NH4NO3 0.0016g Na2HPO4 0.008g
氯化鍶 0.034g 硼酸 0.022g
硅酸鈉 0.004g 氟化鈉 0.0024g
瓊脂 15.0g 蒸餾水 1000ml
pH 7.4-7.8
60. 兔血瓊脂(ATCC 4)
牛心(取其浸液) 500.0g 胰蛋白胨 (Tryptose) 10.0g
NaCl 5.0g 瓊脂 15.0g
蒸餾水 1000ml pH 6.6-7.0
61. 孢子形成培養(yǎng)基 (ATCC 5)
酵母膏 1.0g 牛肉膏 1.0g
胰蛋白胨 2.0g 葡萄糖 10.0g
FeSO4 微量 瓊脂 15.0g
蒸餾水 1000ml pH 7.2
62. Trypticase Soy Agar (ATCC 18)
Trypticase Soy Broth 30.0g 瓊脂 15.0g
蒸餾水 1000ml
63. YPAD (ATCC 109)
酵母膏 10.0g 蛋白胨 20.0g
葡萄糖 20.0g 瓊脂 20.0g
蒸餾水 1000ml pH 7.0
64. SP (ATCC 432)
棉子糖 1.0g 蔗糖 1.0g
半乳糖 1g 可溶性淀粉 5g
水解酪蛋白 2.5g 瓊脂 20.0g
MgSO4.7H2O 0.5g K2HPO4 0.25g
蒸餾水 1000ml pH 自然
65. 胰酪胨大豆酵母浸膏瓊脂 (TSA-YE)
胰蛋白胨 15.5g 大豆蛋白胨 5.0g
NaCl 5.0g 酵母膏 6.5g
瓊脂 15.0g 蒸餾水 1000ml
pH 7.3
66. ATCC 20
蛋白胨 5.0g 酵母膏 15.0g
K2HPO4 3.0g 葡萄糖 2.0g
瓊脂 20.0g 蒸餾水 1000ml
pH 7.3-7.5