Mating Type Factor A and Mating Pheromone Production of Tremella fuciformis

Abstract：Eight strains of Tremella fuciformis were studied. Intrastrain and interstrain cross between single spore isolated from the eight strains indicated that the mating type factor A were biallelic.The effects of culture condition on mating pheromone production by T. fuciformis spores was studied. Single factor and orthogonal tests indicated the optimal culture condition for mating pheromone production was sucrose 20 g/L, (NH4)2SO4 12 mmol/L, MgSO4.*7H2O 0.25 g/L, KH2PO4*3H2O 0.5 g/L,VB1 0.2 mg/L,ZnSO4*7H2O 2 mg/L,CaCl2.*2H2O 0.5 g/L,(NH4)6Mo7O24*4H2O 0.02 mg/L,23 ℃,pH 6.5, 140 r/min. In the optimal culture condition, the highest activity was detected at the culture time of 4 days.The mating pheromone was completely inactivated by the treatment with pepsin, suggesting that the mating pheromone produced by H32 is a kind of peptide.

Keywords：Tremella fuciformis; mating type factor; mating pheromone

銀耳交配型因子及交配信息素的初步研究

盛桂華　 陳立國　 馬愛民　

摘　要：以8個(gè)銀耳菌株為材料,研究交配型因子A在不同菌株中的分布,菌株內(nèi)及菌株間單孢分離物配對(duì)試驗(yàn)結(jié)果表明,此8個(gè)菌株中只有兩個(gè)不同的A因子,與前人報(bào)道的銀耳是限制型雙因子交配系統(tǒng)的報(bào)道是一致的.研究了培養(yǎng)條件對(duì)銀耳孢子產(chǎn)交配信息素的影響, 結(jié)果表明銀耳孢子產(chǎn)交配信息素的較佳培養(yǎng)基為: 蔗糖 20 g/L ,(NH4)2SO4 1.584 g/L(含N 24 mmol/L), MgSO4*7H2O 0.25 g/L , KH2PO4*3H2 O 0.5 g/L , VB1 0.2 mg/L , ZnSO4*7H2O 2 mg/L , CaCl2*2H2O 0.5 g/L,鉬酸銨0.02 mg/L.最佳發(fā)酵條件為培養(yǎng)基pH 6.5,23 ℃,140 r/min,在優(yōu)化培養(yǎng)基和培養(yǎng)條件下,培養(yǎng)4 d發(fā)酵液中交配信息素的活性最高,可達(dá)到12 U/mL,是在基礎(chǔ)培養(yǎng)基中活力的5～6倍.銀耳交配信息素經(jīng)胃蛋白酶處理后,活性完全喪失,由此推測,銀耳交配信息素是一種多肽類物質(zhì).

關(guān)鍵詞：銀耳; 交配型因子; 交配信息素

CLC Number：S646.601

Foundation Item：Supported by a grant from The Center for Microbial Resources, The Institute of Microbiology of Chinese Academy of Sciences (IMCAS)

Author Resume：Sheng Guihua, born in 1976, master of College of Plant Science and Technology,Huazhong Agricultural University, Wuhan 430070, China

Author Unit：盛桂華（華中農(nóng)業(yè)大學(xué)植物科學(xué)與技術(shù)學(xué)院,武漢,430070）　

　　　　　陳立國（華中農(nóng)業(yè)大學(xué)植物科學(xué)與技術(shù)學(xué)院,武漢,430070）　

　　　　　馬愛民（華中農(nóng)業(yè)大學(xué)植物科學(xué)與技術(shù)學(xué)院,武漢,430070）　

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華中農(nóng)業(yè)大學(xué)學(xué)報(bào)

JOURNAL OF HUAZHONG AGRICULTURAL UNIVERSITY

2004 Vol.23 No.1