Polymorphisms of Genetic Characters in Sporulated Monocaryotic Strains of Auricularia Auricula

Abstract：We studied the relationship between the polymorphisms of the mycelial growth vigor and growth rate with the mating type of 38 sporulated monocaryotic strains, esterase patterns polymorphism of 14 sporulated monocaryotic strains, carboxymethyl cellucose(CMC) enzyme relative activities of 10 sporulated monocaryotic strains of Auricularia auricula. The results showed that polymorphism in the mycelial growth vigor and growth rate existed among the sporulated monocaryotic A. auricula strains tested. There was no serious deviation in the 11 ratios between mating type A and mating type a. Ten marker bands of esterase isozyme with different movement rates and 70 bands were screened in 14 tested strains. Compared with parental bicaryotic strain of Au-8, 5 new bands emerged. The CMC enzyme relative activities of 10 sporulated monocaryotic strains of A. auricula were not regular. It was therefore concluded that there was no significant relationship between the mycelial growth vigor and rate with the mating type, and that significant variation was produced among the sporulated monocaryotic strains of A. auricula.

Keywords：Auricularia auricula; sporulated monocaryotic strains; polymorphism; culture characteristics; esterase isozyme; CMC enzyme relative activity

木耳孢子單核菌株遺傳性狀多態(tài)性研究

何培新　 羅信昌　 楊繩桃　 張長鎧　

摘　要：研究了38個木耳孢子單核菌株的菌絲生長勢、菌絲生長速度和其交配型,14個單核菌株的酯酶同工酶以及10個單核菌株的羧甲基纖維素(CMC)酶相對活性,分析了菌絲生長勢和菌絲生長速度與交配型的關(guān)系.結(jié)果表明,供試的木耳孢子單核菌株之間在研究的各遺傳性狀均出現(xiàn)了一定程度的多態(tài)性.在測定的38個孢子單核菌株中,交配型A有21個菌株,交配型a有17個菌株,A∶a=21∶17.經(jīng)χ2檢驗(yàn),兩種交配型未出現(xiàn)1∶1嚴(yán)重偏離.木耳孢子單核菌株菌絲生長勢和菌絲生長速度與其交配型沒有明顯相關(guān)性.在14個單核菌株中,檢測到10種具有不同遷移率的酯酶同工酶標(biāo)記譜帶.與親本雙核菌株相比,出現(xiàn)了5條新的譜帶.10個木耳孢子單核菌株的CMC酶相對活性呈不規(guī)律分布.

關(guān)鍵詞：木耳; 孢子單核菌株; 培養(yǎng)性狀; 酯酶同工酶; CMC酶; 多態(tài)性

CLC Number：S646.6;Q933

Foundation Item：Supported by the Open Project of State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University

Author Resume：He Peixin, male,born in 1970,B.S.,associate professor,Ph.D student in College of Life Science Shandong University,Jinan 250100

Author Unit：何培新（華中農(nóng)業(yè)大學(xué)農(nóng)業(yè)微生物農(nóng)業(yè)部重點(diǎn)實(shí)驗(yàn)室,武漢,430070;山東大學(xué)生命科學(xué)學(xué)院,濟(jì)南,250100）　

　　　　　羅信昌（華中農(nóng)業(yè)大學(xué)農(nóng)業(yè)微生物農(nóng)業(yè)部重點(diǎn)實(shí)驗(yàn)室,武漢,430070）　

　　　　　楊繩桃（華中農(nóng)業(yè)大學(xué)植物保護(hù)系中心實(shí)驗(yàn)室,武漢,430070）　

　　　　　張長鎧（山東大學(xué)生命科學(xué)學(xué)院,濟(jì)南,250100）　

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華中農(nóng)業(yè)大學(xué)學(xué)報

JOURNAL OF HUAZHONG AGRICULTURAL UNIVERSITY

2004 Vol.23 No.1