真菌菌絲體培養(yǎng)和提取DNA方法的改進(jìn)

AN IMPROVED PROTOCOL FOR FUNGAL DNA PREPARATION

何月秋　

　　DNA from fungal mycelia can be inexpensively prepared in a microfuge tube scale.The small scale of the procedure described here makes it possible for one people to conveniently handle up to 60 samples in less than 5 hours and obtain l0～50g of DNA per sample,which is equal to the amount enought for several Southem hybridizations and 500～2500 PCR-based reactions.

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作者單位：何月秋（云南農(nóng)業(yè)大學(xué)植物病理重點(diǎn)實(shí)驗(yàn)室,昆明 650201）