MOLECULAR TAXONOMIC RELATIONSHIPS OF AURICULARIA SPECIES INFERRED FROM RAPD MARKERS

Abstract：Molecular taxonomic relationships of the genus Auricularia, together with outgroup of Tremella, were studied using random amplified polymorphic DNA. Eight species of Auricularia and two species of Tremella were examined. The dendrogram derived from RAPD data by UPGMA suggested that the genera Auricularia and Tremella would be monophyletic. For Auricularia, three distinct genetic groups were recognized: the first included A. Peltata and A. Cornea; the second included all strains of A.auricula; the third included five morphological species of A. Delicata, A. Fuscosuccinea, A. Polytricha, A. Maxima and A. Reticulata. This molecular taxonomy suggests that A.cornea and A. Polytricha would be two different species and not synonymous, and the presence or absence of a medulla in cross-section of the basidiocarps would not indicate whether or not taxa are closely related.

Keywords： Jelly fungi, Molecular taxonomy, Heterobasidiomycetes, Auriculariales

利用RAPD標(biāo)記構(gòu)建木耳屬種間關(guān)系的研究

閆培生　 蔣家慧　 王東昌　 羅信昌　 周啟　

摘　要：利用RAPD標(biāo)記以銀耳屬的2個種為外類群研究了木耳屬8個種的分子系統(tǒng)發(fā)育關(guān)系.根據(jù)UPGMA 構(gòu)建的樹狀圖結(jié)果表明木耳屬(Auricularia)和銀耳屬(Tremella)分別為單一的分類單元.木耳屬的8個種被分成3個明顯不同的組,第一組包括盾形木耳(A.peltata Lloyd)和角質(zhì)木耳(A.cornea Spreng.),第二組包括所有供試的黑木耳(A.auricula Underw.),第三組包括皺木耳(A.delicata Henn.)、琥珀木耳(A.fuscosuccinea Farl.)、毛木耳(A.polytricha Sacc.)、大木耳(A.maxima Y. R. Guo)和網(wǎng)膜木耳(A.reticulata L. J. Li)五個形態(tài)種.種間發(fā)育關(guān)系表明角質(zhì)木耳和毛木耳應(yīng)是兩個不同的種而非同種異名,子實體橫切面中髓層的有無并不表明不同種間親緣關(guān)系的遠(yuǎn)近.

關(guān)鍵詞：膠質(zhì)真菌,分子系統(tǒng)學(xué), 異隔擔(dān)子菌,木耳目

分類號：Q939.96 　文獻(xiàn)標(biāo)識碼：A

文章編號：1007-3515(2002)01-0047-0052

基金項目：Supported by the National Natural Science Foundation of China(國家自然科學(xué)基金資助項目)

作者單位：閆培生（萊陽農(nóng)學(xué)院應(yīng)用真菌研究室,山東萊陽,265200）　

　　　　　蔣家慧（萊陽農(nóng)學(xué)院應(yīng)用真菌研究室,山東萊陽,265200）　

　　　　　王東昌（萊陽農(nóng)學(xué)院應(yīng)用真菌研究室,山東萊陽,265200）　

　　　　　羅信昌（華中農(nóng)業(yè)大學(xué)農(nóng)業(yè)部農(nóng)業(yè)微生物重點實驗室,武漢430070）　

　　　　　周啟（華中農(nóng)業(yè)大學(xué)農(nóng)業(yè)部農(nóng)業(yè)微生物重點實驗室,武漢430070）　

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