分子標(biāo)記在食用蕈菌遺傳育種中的應(yīng)用

APPLICATION OF MOLECULAR MARKERS IN GENETIC AND BREEDING OF EDIBLE MUSHROOM

馬富英　羅信昌　

關(guān)鍵詞：食用蕈菌, 分子標(biāo)記, 遺傳育種

分類號：Q939.96 　文獻標(biāo)識碼：A

文章編號：1007-3515(2002)01-0147-0151

　　分子標(biāo)記是以個體間遺傳物質(zhì)內(nèi)核苷酸序列變異為基礎(chǔ)的遺傳標(biāo)記,是DNA水平遺傳變異的直接反映.與其它幾種遺傳標(biāo)記--形態(tài)標(biāo)記、同工酶標(biāo)記、細胞標(biāo)記相比,分子標(biāo)記具有很多優(yōu)越性:大多數(shù)分子標(biāo)記共顯性遺傳,對隱性的農(nóng)藝性狀的選擇十分便利;基因組變異極其豐富,分子標(biāo)記的數(shù)量幾乎是無限的;在生物發(fā)育的不同階段,不同組織的DNA都可用于標(biāo)記分析;分子標(biāo)記直接揭示來自DNA的變異;表現(xiàn)為中性,不影響目標(biāo)性狀的表達,與不良性狀無必然的連鎖.……

基金項目：國家自然科學(xué)基金資助項目

作者單位：馬富英（華中農(nóng)業(yè)大學(xué)植物保護系,農(nóng)業(yè)部農(nóng)業(yè)微生物重點實驗室,武漢,430070）　

　　　　　羅信昌（華中農(nóng)業(yè)大學(xué)植物保護系,農(nóng)業(yè)部農(nóng)業(yè)微生物重點實驗室,武漢,430070）　

參考文獻：

［1］馬愛民, 潘迎捷, 關(guān)海山, 1998. 雙孢蘑菇原生質(zhì)體同核體及雜交異核體的RAPD分析. 食用菌學(xué)報, 5(3):1～5

［2］王鐳, 譚琦, 陳明杰等, 1997. 從DNA水平上分析香菇不同菌株的遺傳差異. 食用菌學(xué)報, 4(4)：21～24

［3］李英波, 羅信昌, 李濱等, 1995. 香菇菌株的限制性片段長度多態(tài)性. 真菌學(xué)報, 14(3)：209～217

［4］劉國振, 劉振岳, 賈建航等, 1995. 用RAPD方法對平菇、香菇屬間原生質(zhì)體融合子的研究. 遺傳, 17(5):37～40

［5］陳洪, 朱立煌, 徐吉成等, 1995. RAPD標(biāo)記構(gòu)建水稻分子連鎖圖. 植物學(xué)報, 37：677～684

［6］陳作紅, 張志光, 張?zhí)鞎? 2000. 鵝膏菌屬真菌RAPD分析及親緣關(guān)系的研究. 菌物系統(tǒng), 19(1)：51～55

［7］陳明杰, 譚琦, 汪昭月等, 1997. 應(yīng)用RAPD對香菇融合菌株進行遺傳鑒定. 食用菌學(xué)報, 4(4)：17～20

［8］肖在勤, 譚偉, 彭衛(wèi)紅等, 1998. 金針菇及鳳尾菇科間原生質(zhì)體融合研究. 食用菌學(xué)報, 5(1)：6～12

［9］宋思揚, 曾偉, 陳融等, 2000. 一個與雙孢蘑菇子實體品質(zhì)相關(guān)的DNA片段的克隆. 食用菌學(xué)報, 7(3)：11～15

［10］施慶利, 羅信昌, 1994. RAPD技術(shù)在木耳雜交種及原生質(zhì)體融合子鑒定分析中的應(yīng)用. 中國食用菌, 12(6)：3～4

［11］秦國夫, 賀偉, 沈瑞祥, 1996. 中國蜜環(huán)菌生物種的RAPD分析. 真菌學(xué)報, 15(1)：26～33

［12］閻培生, 羅信昌, 周啟, 1999. 木耳屬真菌rDNA特異性擴增片段的RFLP研究. 菌物系統(tǒng), 18(2)：206～213

［13］閻培生, 羅信昌, 周啟, 2000. 利用RAPD技術(shù)對木耳屬菌株進行分類鑒定的研究. 菌物系統(tǒng), 19(1)：29～33

［14］曾偉, 宋思揚, 陳融等, 2000. 應(yīng)用RAPD技術(shù)分析兩個雙孢蘑菇遺傳家系. 微生物學(xué)通報, 27(2):132～134

［15］曾偉, 宋思揚, 王澤生等, 1999. 雙孢蘑菇及大肥菇的種內(nèi)及種間多態(tài)性RAPD分析. 菌物系統(tǒng), 18(1)：55～60

［16］詹才新, 朱蘭寶, 楊新美, 1995. RAPD技術(shù)在金針菇雜交育種中的應(yīng)用. 食用菌學(xué)報, 2(1)：7～12

［17］詹才新, 朱蘭寶, 楊新美, 1997. 利用RAPD技術(shù)評估金針菇種質(zhì)資源. 食用菌學(xué)報, 4(1)：1～7

［18］詹才新, 張引芳, 凌霞芬等, 1998. 中國主栽雙孢蘑菇菌株的DNA多態(tài)性. 食用菌學(xué)報, 5(2)：1～7

［19］Anderson J B, Petsche D M, Smith M L et al., 1987.Restriction fragment length polymorphisms in biological species of Armillaria mellea. Mycologia, 79:69～76

［20］Botstein D, White R L, Skolnick M H et al., 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet, 32: 314～331

［21］Bunyard B A,Nicholson M S, Royse D J, 1994.A systematic assessment of Morchella using RFLP analysis of the 28S ribosomal RNA gene. Mycologia, 86(6): 762-772

［22］Castle A J, Horgen P A, Anderson J B, 1987. Restriction fragment length Polymorphisms in the mushroom Agaricus brunnescens and Agaricus bitorquis. App1 Environ Microbiol, 53(4): 816～822

［23］Donis-Keller H, Green P, Helms C et al., 1987. A genetic linkage map of the human genome. Cell, 51(2): 319～337

［24］Fukuda M, Harada Y, Imahori S et al., 1995. Inheritance of mitochondrial DNA in sexual crosses and protoplast cell fusions in Lentinula edodes. Curr Genet, 27(6): 550～554

［25］Gandeboeuf D, Dupre C, Nicolas P, et al., 1997. Grouping and identification of Tuber Species using RAPD markers. Can J Bot, 75:36～45

［26］Kerrigan R W, Royer J C, Baller L M et al., 1993. Meiotic behavior and linkage relationships in the secondarily homothallic fungus Agaricus bisporus. Genetics, 133:225～236

［27］Khush R S, Becker E, Wach M, 1992. DNA amplification polymorphisms of the cultivated mushroom Agaricus bisporus. App1 Environ Microbiol, 58(9): 2971～2977

［28］Lanfranco L, Myss P, Marzachi G, et al., 1993. DNA Probes for identification of the ectomycorrhizal fungus Tuber magnatum Pico. FEMS Microbiol Lett, 114:245～252

［29］Larraya L M, Perez G, Ritter E, et al., 2000. Genetic linkage map of the edible basidiomycete Pleurotus ostreatus. Appl Environ Microbiol, 66(12): 5290～2300

［30］Loftus M G, Loddler S C, Legg E J, et al., 1995. Molecular mushroom breading. In: Elliott TJ (ed.), Science and Cultivation of Edible Fungi. The Netherlands: Balkema, Rotterdam. 3～9

［31］Matsumoto T, Fukumasa-Nakei Y, 1996. Mitochondrial DNA inheritance in sexual crosses of Pleurotus ostreatus. Curr Genet, 30(6): 549～552

［32］May G, Taylor J W, 1988. Patterns of mating and mitochondrial DNA inheritance in the agaric basidiomycete Coprinus cinereus. Genetics, 118: 213～220

［33］Molina FI, Shen P, Jong S H et al., 1992. Molecular evidence supports the separation of Lentinula edodes from Lentinus and related genera. Can J Bot, 70:2446～2452

［34］Moncalvo J M, Wang H H, Hseu R S, 1987. Phylogenetic relationship in Ganoderma inferred from the internal transcribed spacers and 25S ribosomal DNA sequences. Mycologia, 79 (2): 223～238

［35］Muraguchi H, Kamada T, 1998. The ich1 gene of the mushroom Coprinus cinereus is essential for pileus formation in fruiting. Development, 125(16): 3133～3141

［36］Nicholson M S, Bunyard B A, Royse D J, 1997. .Phylogeny of the genus Lentinula based on ribosomal DNA restriction fragment length polymorphism analysis. Mycologia, 89(3): 400～407

［37］Ospina-Giraldo M D, Collopy P D, Romaine C P et al., 2000. Classification of sequences expressed during the primordial and basidiome stages of the cultivated mushroom Agaricus bisporus. Fungal Genet Biol, 29(2): 81～94

［38］Ramirez L, Muez V, Alfonso M et al., 2001. Use of molecular markers to differentiate between commercial strains of the button mushoom Agaricus bisporus. FEMS Microbiol Lett, 198(1): 45～48

［39］Sagawa I, Tanaka M, Nagata Y, 1992. Discrimination of mushrooms in genus Pleurotus by DNA restriction fragment length polymorphism. J Gen Appl Microbiol, 38:597～603

［40］Sagawa I, Moriyama Y, Yanagi S O et al., 1998. Rapid discrimination of mitochondrial DNA type and use of results to study mitochondrial inheritance in Pleurotus spp. Biosci Biotechnol Biochem, 62(4): 769～772

［41］Sonnerberg A S, de Groot P W, Schaap P J et al., 1996.Isolation of expressed sequence tags of Agaricus bisporus and their assignment to chromosomes. App1 Environ Microbiol, 62(12): 4542～4547

［42］Xu J, Kerrigan R W, Horgen P A et al., 1993. Localization of the mating type gene in Agaricus bisporus. Appl Environ Microbiol, 59:3044～3049

［43］Zeng R, Liu Z T, 1999. Inactivated protoplast electrofusion of edible mushrooms and characterization of intergeneric fusion products. In: Abstracts of 3rd International Conference of Mushroom Biology and Mushroom Products. 51

［44］Zhang Y, Molina F I, 1995. Strain typing of Lentinula edodes by random amplified polymorphic DNA assay. FEMS Microbiol Lett, 131(1): 17～20